Development of an electronic system based on electrochemical sensors and biosensors for the control of biogenic amines


 

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PROJECT WORK PLAN (2012 - 2015)

Romanian version

Year Objectives Activities Results to be delivered
2012 1. Modeling and simulating studies of interaction between biogenic amine compounds and receptor element of sensors and biosensors. 1.1. Modeling of interaction mechanism between biogenic amines and receptor element of sensors in absence and applying a potential

Publication of minimum 2 ISI papers

 

 

Participation to at least 3 scientific conferences
1.2. Modeling of interaction mechanism between biogenic amines and receptor element of biosensors in absence and applying a potential
1.3. Simulation of chemical species dynamics at the surface of sensors and biosensors
1.4. Theoretical study about the influence of some parameters (pH, temperature) on the biosensor response
2. Selection of  sensors and biosensors designs 2.1. Study of commercial sensors designs
2.2. Design of novel sensors
2.3. Use of voltammetry and potentiometry in measurements of sensors and biosensors responses
3. Improvement of sensors qualities and design of novel sensors and biosensors 3.1. Synthesis and characterization of molecular materials
3.2. Synthesis and characterization of conducting polymers
3.3. Fabrication of novel sensors and biosensors: selection of the substrates, selection and application of deposition methods, immobilization of enzymes onto electrodes

3.4. Characterization of sensors and biosensors by spectroscopic (UV-Vis, NIR and IR) and microscopic (SEM, BAM and AFM) techniques

2013 1. Fundamental studies of the interaction between sensitive layer and samples under study 1.1. Study of interactions between biogenic amines and active layer of sensors

Publication of minimum 2 ISI papers

 

Participation to at least 3 scientific conferences
1.2. Study of interactions between biogenic amines and active layer of biosensors
1.3. Study of enzyme kinetics of biosensors
1.4. Comparison of experimental data with those obtained in modeling and simulation studies
2. Data treatment and interpretation of the results 2.1 Establishment of methods for data analysis. Exploratory analysis
2.2 Pre-processing of experimental data
2.3 Application of Principal Component Analysis method for processing of experimental data
2.4 Application of classification methods (PLS-DA and SIMCA) for processing of experimental data

2.5 Establishment of correlations between sensors and biosensors signals and the results of physico-chemical analysis or sensory

2014 1. Test of the sensing capabilities of the sensors and biosensors   1.1. Study of the selectivity of sensors and biosensors     

Publication of minimum 2 ISI papers

 

Participation to at least 2 scientific conferences
1.2. Study of the stability of sensors and biosensors
1.3. Study of the reproducibility of sensors and biosensors
1.4. Study of the lifetime of sensors and biosensors
1.5. Determination of the detection limits of sensors and biosensors
1.6. Determination of the response time of sensors and biosensors
1.7. Determination of the reversibility and the recovery of sensors and biosensors
2015 1. Test of sensors and biosensors in real samples. Applicative studies 1.1. Selecting of sensors and biosensors arrays for applications in real samples

Publication of minimum 2 ISI papers

 

Participation to at least 2 scientific conferences
1.2. Selection of the methods for data treatment
1.3. Analysis of biogenic amines in meat products, cheeses and fermented beverages
2016 1. Test of sensors and biosensors in real samples. Applicative studies 1.4. Monitoring of food freshness

Publication of minimum 2 ISI papers

 

Participation to at least 2 scientific conferences

1.5. Analysis of biogenic amines in fruits

1.6. Analysis of biogenic amines in clinical samples
1.7. Validation of the systems by the establishment of correlations between the results obtained with sensors and biosensors and the results of physico-chemical, sensory, biochemical and medical analyses

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